| 1. | Cloning of soluble interleukin 4 receptor gene and construction of adenovirus shuttle vector
4受体基因克隆及腺病毒载体的构建 |
| 2. | After its sequence was determined , the mutant gene was cloned into a shuttle vector pnov - r and transformed into br - deficient halobacteria ( l33 )
突变后的蛋白经生物信息学软件分析了和野生型br的不同。 tyr79 argbr突变体的光电特性还要进一步分析和测试。 |
| 3. | The expression plasmid called psugv - badfe was constructed by inserting ba - dfe gene into e . coli - b . subtilis shuttle vector psugv4 and the
将ba0fe基因克隆到大肠杆菌一枯草杆菌穿梭载体psugv4中，得到重组质粒psugv badfe ，然后转化到枯草杆菌wb600中进行了分泌表达。 |
| 4. | Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
解离载体的构建和性能利用来源于不同质粒上的质粒复制起始区ori44和ori1030构建了4个解离载体。 |
| 5. | Therefore , the 4 . 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm , ) and resulted in a cloned strain bl - 3 . this strain could express only 130kda protein
它不仅对苏云金芽胞杆菌工程菌的构建具有现实意义，而且对其它基因高表达研究将产生积极影响，具有一定的理论意义和潜在的应用价值 |
| 6. | With the aim of increasing the expression and stability of mtxl , the mtxl gene originated from b . sphaericus ssii1 was cloned to a shuttle vector pbu4 . two recombinant plasmids pmt9 and pmt4 were obtained , with the inserted fragments in the opposite orientations
为了提高mtx1杀蚊毒素蛋白的表达量和稳定性，本文将来源于球形芽孢杆菌b . sss - 1的mtx1毒素基因克隆至穿梭载体pbu4上，得到mtx1插入方向相反的重组质粒pmt9和pmt4 。 |
| 7. | 3 . the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ) , p3a was spliced with the cry1c gene , then inserted into the shuttle vector pht304 , and then recombinated plasmid pbmb827 was obtained . after transferring pbmb827 into strain ybt - 1520 , it was surprising that the transformants had almost no potency against all lepidopteran larvae tested
3非依赖芽胞形成icp的cry3a启动子( p _ ( 3a ) )对野生菌株特性的影响带p _ ( 3a )和cry1c基因的重组质粒pbmb827转入ybt - 1520 ，转化子对所测昆虫的毒力下降非常明显，芽胞和晶体也很难脱落。 |
| 8. | A 1 . 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e . coli / streptomyces shuttle vector with conjugation function ( containing orit gene ) . as a result of above procedures , a recombinant plasmid pid03 was obtained
将1 . 5kb的安普霉素抗性基因片段插入到aved基因中的nrui酶切位点，再将此灭活的aved基因片段插入到具有接合转移功能(含有orit基因)的链霉菌?大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pid03 。 |
| 9. | The sacvgb gene was then cloned into e . coli - - b . sublilis shuttle vector psugv4 and a recombinant plasmid named psu - sacvgb was constructed . after transformation with b . sublilis wb600 , the transformants successfully produced vhb intracellularly . the influence of vhb on b . subtilis is under investigation
将sacvgb基因克隆到大肠杆菌-枯草杆菌穿梭载体psugv4中，获得重组表达质粒psu - sacvgb ，然后转化枯草杆菌wb600 ，转化子经2蔗糖诱导，该基因在枯草杆菌中表达。 |
| 10. | A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。 |
